Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR
نویسندگان
چکیده
منابع مشابه
Detection of genetically modified maize in processed foods sold commercially in Iran by qualitative PCR
Detection of genetically modified organisms (GMOs) in food is an important issue for all the subjects involved in food control and customer’s right. Due to the increasing number of GMOs imported to Iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. In this study, following extraction of genomic DNA ...
متن کاملDetection of genetically modified maize in processed foods sold commercially in Iran by qualitative PCR
Detection of genetically modified organisms (GMOs) in food is an important issue for all the subjects involved in food control and customer’s right. Due to the increasing number of GMOs imported to Iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. In this study, following extraction of genomic DNA ...
متن کاملQualitative and quantitative PCR methods for detection of three lines of genetically modified potatoes.
Qualitative and quantitative polymerase chain reaction (PCR) methods have been developed for the detection of genetically modified (GM) potatoes. The combination of specific primers for amplification of the promoter region of Cry3A gene, potato leafroll virus replicase gene, and potato virus Y coat protein gene allows to identify each line of NewLeaf, NewLeaf Y, and NewLeaf Plus GM potatoes. Mu...
متن کاملDetection of genetically modified crops by combination of multiplex PCR and low-density DNA microarray.
OBJECTIVE To develop a technique for simultaneous detection of various target genes in Roundup Ready soybean by combining multiplex PCR and low-density DNA microarray. METHODS Two sets of the multiplex PCR system were used to amplify the target genes in genetically modified (GM) soybean. Seventeen capture probes (PCR products) and 17 pairs of corresponding primers were designed according to t...
متن کاملdetection of genetically modified maize in processed foods sold commercially in iran by qualitative pcr
detection of genetically modified organisms (gmos) in food is an important issue for all the subjects involved in food control and customer’s right. due to the increasing number of gmos imported to iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. in this study, following the extraction of genomic ...
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ژورنال
عنوان ژورنال: Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
سال: 2014
ISSN: 1211-8516,1211-8516
DOI: 10.11118/actaun200957050313